Genome Dynamics Laboratory

Maeshima Group

3D-organization and dynamics of human genome

Faculty

MAESHIMA, Kazuhiro
Professor

kmaeshim@nig.ac.jp

researchmap

 Lab HP

Research Summary

Our research interest is to know how a long string of human genome is three-dimensionally organized in the cell, and how the human genome is read out for cellular proliferation, differentiation and development. For this purpose, we are using a unique combination of molecular cell biology and biophysics, such as live-cell single molecule imaging, superresolution imaging, genomics and computational simulation.

Human genome chromatin consists of irregularly folded 10-nm fibers and forms numerous chromatin domains in the cell nuclei. The chromatin domain dynamically behaves like “liquid”. NPC, nuclear pore complex; NE, nuclear envelope.

Selected Publications

  • Iida S, Ide S, Tamura S, Sasai M, Tani T, Goto T, Shribak M, Maeshima K. Orientation-independent-DIC imaging reveals that a transient rise in depletion attraction contributes to mitotic chromosome condensation. Proc Natl Acad Sci U S A. 2024 Sep 3;121(36):e2403153121.
  • Hibino K, Sakai Y, Tamura S, Takagi M, Minami K, Natsume T, Shimazoe MA,Kanemaki MT, Imamoto N, Maeshima K. Single-nucleosome imaging unveils that condensins and nucleosome-nucleosome interactions differentially constrain chromatin to organize mitotic chromosomes. Nat Commun. 2024 Aug 21;15(1):7152.
  • Maeshima K, Iida S, Shimazoe MA, Tamura S, Ide S. Is euchromatin really open in the cell? Trends Cell Biol. 2024 Jan;34(1):7-17.
  • Nozaki T, Shinkai S, Ide S, Higashi K, Tamura S, Shimazoe MA, Nakagawa M,Suzuki Y, Okada Y, Sasai M, Onami S, Kurokawa K, Iida S, Maeshima K. Condensed but liquid-like domain organization of active chromatin regions in living human cells. Sci Adv. 2023 Apr 5;9(14):eadf1488.